Anti-mildew Activity of Methanolic Extract from Selected Medicinal Plants against Plasmopara Halstedii (farl.) Berl. & De Toni Incitant of Sunflower Downy Mildew Disease
نویسندگان
چکیده
Methanolic extracts of eight medicinal plants were screened for their anti-mildew activity against Plasmopara halstedii, incitant of downy mildew of sunflower. Extracts of Morinda citrifolia, Zingiber officinale, and Tinospora cordifollia were found to be best in reducing the sporulation of P. halstedii in both the whole leaf and leaf disc method. Further; the phytochemical analysis of these extracts revealed the presence of alkaloids, glycosides, steroids, flavonoids, reducing sugars, phenolics, tannins, saponins and terpenoids. Different compounds from the plant extracts were partially purified through preparative thin layer chromatography and analyzed for their antimildew activity following leaf disc method. The assay confirmed that bioactive compound from M. citrifolia (Rf, 0.47), Z. officinale (Rf, 0.6) and T. cordifollia (Rf, 0.12) were antimildew in nature. In vitro sporangiosporicidal assay revealed that, partially purified antimildew compounds significantly reduced the viable sporangiospores and also inhibited the release of sporangiospores from sporangia.
منابع مشابه
RXLR and CRN Effectors from the Sunflower Downy Mildew Pathogen Plasmopara halstedii Induce Hypersensitive-Like Responses in Resistant Sunflower Lines
Plasmopara halstedii is an obligate biotrophic oomycete causing downy mildew disease on sunflower, Helianthus annuus, an economically important oil crop. Severe symptoms of the disease (e.g., plant dwarfism, leaf bleaching, sporulation and production of infertile flower) strongly impair seed yield. Pl resistance genes conferring resistance to specific P. halstedii pathotypes were located on sun...
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